mouse anti-human il2 antibody (PeproTech)

PeproTech mouse anti-human il2 antibody

CIRB a novel cytokine-cytokine receptor chimera A, Diagram of the Human <t>IL2</t> and the chimera IL2 fused with receptor IL2Rβ (CIRB) in lentiviral constructs. A Linker (L) composed of the cMyc tag (EQKLISEEDL) and a fragment of the extracellular domain of IL2 receptor alpha (EMETSQFPGEEKPQASPEGRPESETSC), joins IL2 and its receptor IL2Rβ. B, detection by anti-cMyc monoclonal antibody of CIRB expression at the surface of transiently transfected HEK293 cells and C, in lentivirus transduced NK92 cells. D, CD122 expression detected using an anti-CD122 monoclonal antibody, which recognizes the native IL2Rβ (CD122) as well as the chimera. CD122 is present in NK92IL2 cells and as expected, much higher in NK92CIRB due to the additional expression of CIRB. Expression was higher than the background detected in the absence of antibody (Neg). E, CIRB was further detected by western blot using 40ug cell lysates and monoclonal anti-human IL2. CIRB is heavily glycosylated. F, RT-PCR detection of IL2 and CIRB transcripts in NK92IL2 and NK92CIRB. Primers sequences in Supplementary Table S1. G, Only NK92IL2 cells secrete soluble IL2 that could support the growth of co-cultured NK92 cells. Experiment done in six-well plate with inserts containing 100K cells of bystander NK92 in the lower chamber and 100K cells of NK92IL2, NK92CIRB, NK92 or NK92 in the presence of IL2 (20IU/ml)

Mouse Anti Human Il2 Antibody, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-human+il-6+mab/pmc06204197-83-21-25?v=PeproTech
Average 90 stars, based on 1 article reviews

mouse anti-human il2 antibody - by Bioz Stars, 2026-08

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mouse anti–human il-6 mab (Schering-Plough corporation)

Schering-Plough corporation mouse anti–human il-6 mab

<t>Cytokine</t> production by different populations of blood cells stimulated with HSV. Cells were stimulated with HSV for 24 h, and cytokine concentrations in the supernatants were measured by ELISA. PBMC, total PBMCs; Mono, monocytes; CD11c + DC, FACS-sorted CD11c + lin − immature DCs; IPC, FACS ® -sorted CD4 + CD11c − lin − cells. Error bars indicate SD. The data shown are representative of four experiments.

Mouse Anti–Human Il 6 Mab, supplied by Schering-Plough corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-human+il-6+mab/pmc02193254-25-47-51?v=Schering-Plough+corporation
Average 90 stars, based on 1 article reviews

mouse anti–human il-6 mab - by Bioz Stars, 2026-08

90/100 stars

Buy from Supplier

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CIRB a novel cytokine-cytokine receptor chimera A, Diagram of the Human IL2 and the chimera IL2 fused with receptor IL2Rβ (CIRB) in lentiviral constructs. A Linker (L) composed of the cMyc tag (EQKLISEEDL) and a fragment of the extracellular domain of IL2 receptor alpha (EMETSQFPGEEKPQASPEGRPESETSC), joins IL2 and its receptor IL2Rβ. B, detection by anti-cMyc monoclonal antibody of CIRB expression at the surface of transiently transfected HEK293 cells and C, in lentivirus transduced NK92 cells. D, CD122 expression detected using an anti-CD122 monoclonal antibody, which recognizes the native IL2Rβ (CD122) as well as the chimera. CD122 is present in NK92IL2 cells and as expected, much higher in NK92CIRB due to the additional expression of CIRB. Expression was higher than the background detected in the absence of antibody (Neg). E, CIRB was further detected by western blot using 40ug cell lysates and monoclonal anti-human IL2. CIRB is heavily glycosylated. F, RT-PCR detection of IL2 and CIRB transcripts in NK92IL2 and NK92CIRB. Primers sequences in Supplementary Table S1. G, Only NK92IL2 cells secrete soluble IL2 that could support the growth of co-cultured NK92 cells. Experiment done in six-well plate with inserts containing 100K cells of bystander NK92 in the lower chamber and 100K cells of NK92IL2, NK92CIRB, NK92 or NK92 in the presence of IL2 (20IU/ml)

Journal: Cancer research

Article Title: Tethering IL2 to its receptor IL2Rβ enhances anti-tumor activity and expansion of natural killer NK92 cells

doi: 10.1158/0008-5472.CAN-17-1007

Figure Lengend Snippet: CIRB a novel cytokine-cytokine receptor chimera A, Diagram of the Human IL2 and the chimera IL2 fused with receptor IL2Rβ (CIRB) in lentiviral constructs. A Linker (L) composed of the cMyc tag (EQKLISEEDL) and a fragment of the extracellular domain of IL2 receptor alpha (EMETSQFPGEEKPQASPEGRPESETSC), joins IL2 and its receptor IL2Rβ. B, detection by anti-cMyc monoclonal antibody of CIRB expression at the surface of transiently transfected HEK293 cells and C, in lentivirus transduced NK92 cells. D, CD122 expression detected using an anti-CD122 monoclonal antibody, which recognizes the native IL2Rβ (CD122) as well as the chimera. CD122 is present in NK92IL2 cells and as expected, much higher in NK92CIRB due to the additional expression of CIRB. Expression was higher than the background detected in the absence of antibody (Neg). E, CIRB was further detected by western blot using 40ug cell lysates and monoclonal anti-human IL2. CIRB is heavily glycosylated. F, RT-PCR detection of IL2 and CIRB transcripts in NK92IL2 and NK92CIRB. Primers sequences in Supplementary Table S1. G, Only NK92IL2 cells secrete soluble IL2 that could support the growth of co-cultured NK92 cells. Experiment done in six-well plate with inserts containing 100K cells of bystander NK92 in the lower chamber and 100K cells of NK92IL2, NK92CIRB, NK92 or NK92 in the presence of IL2 (20IU/ml)

Article Snippet: Western Blot Phophorylated Stat 5 was detected using rabbit anti-STAT5 Phospho (Tyr694) antibody (BioLegend) while the chimera CIRB was detected by mouse anti-human IL2 antibody (Peprotech), in 40ug cell lysates obtained using Ripa lysis buffer (Santa Cruz Biotech).

Techniques: Construct, Expressing, Transfection, Western Blot, Reverse Transcription Polymerase Chain Reaction, Cell Culture

Impact of TGFβ1, IL4 and Dexamethasone on NK cells. A, 64×103 NK92IL2 and B, NK92CIRB cells were plated with TGFβ1 (10ng/ml), IL4 (10ng/ml), or Dex (1uM) for 6 days of growth. Viable cells were counted using Trypan blue exclusion assay. Data are presented as live cell count (105 cells/ml), mean + SE values for triplicate samples. C, NK92, NK92IL2 and NK92CIRB cell lines exposed for 24 hours to TGFβ1 (20ng/ml■), Dex (0.5uM▲), or no drug (UT●). NK 92 cells were incubated with IL2 at 20IU/ml. Cancer cells (32 ×103 cells), were then added to NK cells at E/T ratio of 2:1, then incubated for 4 days. Cancer cells viability was determined using a crystal violet extraction assay. Data are presented as mean percentage cell number relative to NK cells-free controls (UT). D, Natural cytotoxicity receptors and AnnexinV expression in NK92IL2 and NK92CIRB treated with dex1uM or TGFβ1 10ng/ml.

Journal: Cancer research

Article Title: Tethering IL2 to its receptor IL2Rβ enhances anti-tumor activity and expansion of natural killer NK92 cells

doi: 10.1158/0008-5472.CAN-17-1007

Figure Lengend Snippet: Impact of TGFβ1, IL4 and Dexamethasone on NK cells. A, 64×103 NK92IL2 and B, NK92CIRB cells were plated with TGFβ1 (10ng/ml), IL4 (10ng/ml), or Dex (1uM) for 6 days of growth. Viable cells were counted using Trypan blue exclusion assay. Data are presented as live cell count (105 cells/ml), mean + SE values for triplicate samples. C, NK92, NK92IL2 and NK92CIRB cell lines exposed for 24 hours to TGFβ1 (20ng/ml■), Dex (0.5uM▲), or no drug (UT●). NK 92 cells were incubated with IL2 at 20IU/ml. Cancer cells (32 ×103 cells), were then added to NK cells at E/T ratio of 2:1, then incubated for 4 days. Cancer cells viability was determined using a crystal violet extraction assay. Data are presented as mean percentage cell number relative to NK cells-free controls (UT). D, Natural cytotoxicity receptors and AnnexinV expression in NK92IL2 and NK92CIRB treated with dex1uM or TGFβ1 10ng/ml.

Techniques: Trypan Blue Exclusion Assay, Cell Counting, Incubation, Expressing

NK92, NK92IL2 and NK92CIRB cell lines phenotype. A, Flow cytometry shows the increased cell surface density of CD16 in NK92IL2 and NK92CIRB and the low expression of CD25 in NK92CIRB. B, Flow cytometry confirms the lack of expression of CD16 in NK92 and NK92-MI cell lines. C, shows the lack of expression of CD16 in NK92 activated with 100 or 1000IU/m of glycosylated (IL2G) and non glycosylated IL2. D, Human primary NK cells (hNK) phenotypic expression of NKG2D, CD25 and CD16 in comparison to NK92, NK92IL2 and NK92CIRB. E, direct cytotoxicity and ADCC activity mediated by effector NK92, NK92IL2 and NK92CIRB cells against HER2 positive BT474 cell line at E/T ratio of 2:1 when incubated with Trastuzumab (1ug/ml). Data are presented as cell number percentage relative to NK cells-free controls, mean + SE values for triplicate samples. Statistical differences were determined by one-way Anova test ((*P<0.05).

Journal: Cancer research

Article Title: Tethering IL2 to its receptor IL2Rβ enhances anti-tumor activity and expansion of natural killer NK92 cells

doi: 10.1158/0008-5472.CAN-17-1007

Figure Lengend Snippet: NK92, NK92IL2 and NK92CIRB cell lines phenotype. A, Flow cytometry shows the increased cell surface density of CD16 in NK92IL2 and NK92CIRB and the low expression of CD25 in NK92CIRB. B, Flow cytometry confirms the lack of expression of CD16 in NK92 and NK92-MI cell lines. C, shows the lack of expression of CD16 in NK92 activated with 100 or 1000IU/m of glycosylated (IL2G) and non glycosylated IL2. D, Human primary NK cells (hNK) phenotypic expression of NKG2D, CD25 and CD16 in comparison to NK92, NK92IL2 and NK92CIRB. E, direct cytotoxicity and ADCC activity mediated by effector NK92, NK92IL2 and NK92CIRB cells against HER2 positive BT474 cell line at E/T ratio of 2:1 when incubated with Trastuzumab (1ug/ml). Data are presented as cell number percentage relative to NK cells-free controls, mean + SE values for triplicate samples. Statistical differences were determined by one-way Anova test ((*P<0.05).

Techniques: Flow Cytometry, Expressing, Activity Assay, Incubation

NK92IL2 and NK92CIRB cells evaluation in vivo. A, When U251 tumor volume reached ~160mm3, non-irradiated NK92IL2 and NK92CIRB cells (107 cells in 200ul per mouse) were injected into mice (arrows), via the tail vein. A second injection of non-irradiated NK cells (5×106 cells) was carried out 4 days later. Tumor sizes were monitored until 31 days post tumor implantation. B, 17 days after the last NK cells injection, animals were sacrificed and blood was collected from 3 animals in each group. Blood samples (0.5ml) were processed and analyzed by flow cytometry using human specific marker CD45 and the mCherry fluorescence marker, which is co-expressed with IL2 or CIRB in NK92IL2 and NK92CIRB, respectively. NK92CIRB cells detected (circled). C, Cell survival of NK cells irradiated in vitro at 10Gy (0.83gy for 12 min) and then plated in complete NK92 media was determined using Trypan Blue exclusion every 24 hours. The survival advantage of NK92CIRB cells was statistically significant at days 1 and 2 (One-way Anova test *P<0.05). D, anti-tumor efficacy of irradiated NK92IL2 and NK92CIRB cells in vivo against PC-3 tumors grown in 5 week-old male Nod/Scid mice. When tumor size reached ~200 mm3, NK cells were irradiated with 500cGy were administered as four weekly injections of 15×106 cells in 200ul per mouse, via the tail vein (arrows). After the last NK92CIRB cells injection, a significant tumor growth delay of 17 days was recorded (**P<0.01), comparatively to the untreated group. NK92IL2 treated group tumors produced a tumor delay of only 7 days (*P<0.05). Statistical differences were determined using One-way Anova test.

Journal: Cancer research

Article Title: Tethering IL2 to its receptor IL2Rβ enhances anti-tumor activity and expansion of natural killer NK92 cells

doi: 10.1158/0008-5472.CAN-17-1007

Figure Lengend Snippet: NK92IL2 and NK92CIRB cells evaluation in vivo. A, When U251 tumor volume reached ~160mm3, non-irradiated NK92IL2 and NK92CIRB cells (107 cells in 200ul per mouse) were injected into mice (arrows), via the tail vein. A second injection of non-irradiated NK cells (5×106 cells) was carried out 4 days later. Tumor sizes were monitored until 31 days post tumor implantation. B, 17 days after the last NK cells injection, animals were sacrificed and blood was collected from 3 animals in each group. Blood samples (0.5ml) were processed and analyzed by flow cytometry using human specific marker CD45 and the mCherry fluorescence marker, which is co-expressed with IL2 or CIRB in NK92IL2 and NK92CIRB, respectively. NK92CIRB cells detected (circled). C, Cell survival of NK cells irradiated in vitro at 10Gy (0.83gy for 12 min) and then plated in complete NK92 media was determined using Trypan Blue exclusion every 24 hours. The survival advantage of NK92CIRB cells was statistically significant at days 1 and 2 (One-way Anova test *P<0.05). D, anti-tumor efficacy of irradiated NK92IL2 and NK92CIRB cells in vivo against PC-3 tumors grown in 5 week-old male Nod/Scid mice. When tumor size reached ~200 mm3, NK cells were irradiated with 500cGy were administered as four weekly injections of 15×106 cells in 200ul per mouse, via the tail vein (arrows). After the last NK92CIRB cells injection, a significant tumor growth delay of 17 days was recorded (**P<0.01), comparatively to the untreated group. NK92IL2 treated group tumors produced a tumor delay of only 7 days (*P<0.05). Statistical differences were determined using One-way Anova test.

Techniques: In Vivo, Irradiation, Injection, Tumor Implantation, Flow Cytometry, Marker, Fluorescence, In Vitro, Produced

Cytokine production by different populations of blood cells stimulated with HSV. Cells were stimulated with HSV for 24 h, and cytokine concentrations in the supernatants were measured by ELISA. PBMC, total PBMCs; Mono, monocytes; CD11c + DC, FACS-sorted CD11c + lin − immature DCs; IPC, FACS ® -sorted CD4 + CD11c − lin − cells. Error bars indicate SD. The data shown are representative of four experiments.

Journal: The Journal of Experimental Medicine

Article Title: Natural Interferon α/β–Producing Cells Link Innate and Adaptive Immunity

doi:

Figure Lengend Snippet: Cytokine production by different populations of blood cells stimulated with HSV. Cells were stimulated with HSV for 24 h, and cytokine concentrations in the supernatants were measured by ELISA. PBMC, total PBMCs; Mono, monocytes; CD11c + DC, FACS-sorted CD11c + lin − immature DCs; IPC, FACS ® -sorted CD4 + CD11c − lin − cells. Error bars indicate SD. The data shown are representative of four experiments.

Article Snippet: IPCs were cultured for 3 d with 10 6 PFU/ml HSV, 10 ng/ml IL-3 (R&D Systems), 500 IU/ml IFN-α 2b (Schering-Plough), 10 ng/ml TNF-α (R&D Systems), 10 ng/ml IL-6 (DNAX), 20 μg/ml mouse anti–human TNF-α mAb (MP9-20A4; a gift from J. Abrams, DNAX), or 20 μg/ml mouse anti–human IL-6 mAb (MQ2-39C3; DNAX).

Techniques: Enzyme-linked Immunosorbent Assay

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